READ ME text document

OBJECTIVES
Gather and analyze phenotypic data related to water use efficiency (WUE) in sagebrush seedlings.

PERSONNEL
Carlos Dave Dumaguit, Boise State University, Boise, ID, USA
carlosdavedumagu@boisestate.edu

Principal Investigator: Sven Buerki, Ph.D., Boise State University, Boise, ID, USA
svenbuerki@boisestate.edu

Stephanie Rood, undergraduate student intern

Walker Morales, undergraduate student intern

FUNDING
US National Science Foundation and Idaho EPSCoR: OIA-1757324

DATA COLLECTION
All data was collected in the Summer of 2021. Specific data collection methods can be found in data specific README files for stomata, xylem, and leaf area data.

Carbon isotope data exported to:
Stable Isotope Laboratory
Boise State University, Department of Geosciences
1910 University Drive, Boise, ID 83725-1247
Method notes: run on a Costech EA coupled to a Thermo Delta V Plus mass spectrometer; data normalized to USGS 40 (d15N= -4.52 ‰, d13C= -26.39 ‰) and USGS 41a (d15N= 47.55 ‰, d13C= 36.55 ‰) 

STANDARDS
SI - Systeme International (Metric system)

SOFTWARE(S)
R Core Team (2022). R: A language and environment for statistical computing. R Foundation for Statistical
Computing, Vienna, Austria. URL https://www.R-project.org/.
Rasband, W.S., ImageJ, U. S. National Institutes of Health, Bethesda, Maryland, USA, https://imagej.nih.gov/ij/, 1997-2018. 

DATA STRUCTURE
Root folder: 'https://www.northwestknowledge.net/data/92558821-2d5a-4e43-8a96-086c77e564c9'
	'./Stomata/' - This folder contains RAW DATA in curated form for further compilation and analysis of stomata charcteristics.
	'./Xylem/' - This folder contains RAW DATA in curated form for further compilation and analysis of xylem vessel characteristics.
	'./Carbon_isotope/' - Contains raw and cleaned (csv) carbon isotope data.
	'./Leaves_data/' - Contains the full pipeline for analysis of leaf area and comparing at the population level, created by Sven Buerki, Ph.D.

PROCESSING PROCEDURES/PROTOCOLS

KNOWN PROBLEMS/LIMITATIONS

CITATION INSTRUCTIONS
Dumaguit CDC, Buerki S. 2023. Sagebrush greenhouse water use efficiency GxE phenotypic data [dataset]. University of Idaho. https://doi.org/10.7923/jk5q-6876 

IP & LICENSING
Creative Commons Attribution 4.0 International ([CC-BY 4.0])(https://creativecommons.org/licenses/by/4.0/legalcode)